MMP-2、Fhit、RECK、VEGF在喉癌组织中的表达及相关性分析

目的:探讨基质金属蛋白酶-2(MMP-2)、脆性组氨酸三联体基因(Fhit)、逆转录诱导蛋白基因(RECK)、血管内皮细胞生长因子(VEGF)在喉癌组织中的表达及其相关性。方法:选取2011年1月到2016年12月在陕西省人民医院接受治疗的喉癌患者80例,收集其手术中切除的喉癌组织和癌旁组织,另收集40例喉癌组织切除外缘的正常喉粘膜组织。比较喉癌组织、癌旁组织、正常喉粘膜组织中MMP-2、Fhit、RECK、VEGF的表达,分析喉癌组织中MMP-2、Fhit、RECK、VEGF的表达与临床病理特征的关系,并分析四个指标的相关性。结果:喉癌组织中MMP-2、VEGF表达明显高于癌旁组织和正常喉粘膜组织,Fhit、RECK表达明显低于癌旁组织和正常喉粘膜组织(P0.05)。喉癌组织中MMP-2的表达与淋巴结转移、临床分期、分化程度有关(P0.05);Fhit、RECK的表达与淋巴结转移、临床分期有关(P0.05);VEGF的表达与淋巴结转移有关(P0.05)。喉癌组织中MMP-2的表达水平与Fhit、RECK呈负相关(P0.05),与VEGF呈正相关(P0.05);Fhit与RECK呈正相关(P0.05),与VEGF呈负相关(P0.05);RECK与VEGF呈负相关(P0.05)。结论:在喉癌组织中MMP-2、Fhit、RECK、VEGF均存在异常表达;其相互影响,可能共同参与了喉癌的发生、发展。

Expression and Correlation Analysis of MMP-2, Fhit, RECK and VEGF in Laryngeal Cancer Tissues

ABSTRACT Objective: To explore the expression and correlation of MMP-2, Fhit, RECK and VEGF in laryngeal cancer tissues. Methods: 80 cases of laryngeal cancer who were treated in Shaanxi People''s Hospital from January 2011 to December 2016 were selected, the laryngeal cancer tissues and the paracancerous tissues were collected, and 40 cases of normal laryngeal mucosa tissues in excision margin of laryngeal carcinoma were collected. The expression of MMP-2, Fhit, RECK and VEGF in laryngeal cancer tissues, paracancerous tissues and normal laryngeal mucosa tissues were compared, the expression of MMP-2, Fhit, RECK and VEGF in laryngeal cancer tissues and its relationship with clinicopathological features were analysised, and the correlation of four indexes were analyzed. Results: The expression of MMP-2 and VEGF in laryngeal cancer tissues were significantly higher than those in paracancerous tissues and normal laryngeal mucosa tissues, the expression of Fhit and RECK were significantly lower than those in paracancerous tissues and normal laryngeal mucosa tissues(P<0.05). The expression of MMP-2 in laryngeal cancer tissues was related to lymph node metastasis, clinical stage and degree of differentiation(P<0.05); The expression of Fhit and RECK were related to lymph node metastasis and clinical stage(P<0.05); The expression of VEGF was related to lymph node metastasis (P<0.05). The expression of MMP-2 was negatively correlated with Fhit and RECK in laryngeal cancer tissues (P<0.05), and positively correlated with VEGF (P<0.05); Fhit was positively correlated with RECK (P<0.05), and negatively correlated with VEGF (P<0.05); RECK was negatively correlated with VEGF(P<0.05). Conclusion: The expression of MMP-2, Fhit, RECK and VEGF in laryngeal carcinoma are abnormal, they interact with each other, may be involve in the occurrence and development of laryngeal cancer.