乳腺导管原位癌和浸润性导管癌中MMP-7、VEGF及E-cad的表达及临床意义

目的:探讨乳腺导管原位癌(DCIS)和浸润性导管癌(IDC)中基质金属蛋白酶-7(MMP-7)、血管内皮生长因子(VEGF)及钙黏附素E(E-cad)的表达及临床意义。方法:选取2012年1月-2017年8月期间鄂东医疗集团黄石市中心医院乳甲外科的DCIS石蜡包埋标本(DCIS组)59例,IDC石蜡包埋标本(IDC组)32例,另选取同时期正常乳腺组织标本20例为对照组,检测各组MMP-7、VEGF及E-cad的表达情况,并分析MMP-7、VEGF及E-cad的阳性表达率与DCIS、IDC患者临床病理特征的关系,采用Pearson相关性分析MMP-7、VEGF与E-cad之间的相关性。结果:DCIS组、IDC组的MMP-7、VEGF阳性表达率高于对照组,E-cad的强阳性表达率低于对照组(P0.05),DCIS组与IDC组之间的MMP-7、VEGF、E-cad阳性表达率比较差异无统计学意义(P0.05)。MMP-7、VEGF及E-cad的阳性表达率均与患者的年龄、肿瘤大小无关(P0.05),临床分期为Ⅱ-Ⅲ期、中/低分化程度、有淋巴结转移患者的MMP-7、VEGF的阳性表达率高于临床分期为Ⅰ期、高分化程度、无淋巴结转移患者(P0.05),中/低分化程度、有淋巴结转移患者的E-cad的阳性表达率低于高分化程度、无淋巴结转移患者(P0.05)。经Pearson相关性分析显示,MMP-7与VEGF存在正相关关系(r=0.362,P=0.038),MMP-7、VEGF均与E-cad无显著相关性(r=0.071、0.024,P=0.057、0.089)。结论:DCIS和IDC中MMP-7、VEGF表达较高,E-cad表达较低,且与患者临床分期、分化程度、淋巴结转移有关,临床上可以通过检查MMP-7、VEGF、E-cad的表达来评估乳腺癌的发生及发展。

Expressions and Clinical Significance of MMP-7, VEGF and E-cad in Breast Ductal Carcinoma in Situ and Invasive Ductal Carcinoma

ABSTRACT Objective: To investigate the expressions and clinical significance of Matrix metalloproteinase -7 (MMP-7), vascular endothelial growth factor (VEGF) and Calcium adherent E (E-cad) in breast ductal carcinoma in situ (DCIS) and invasive ductal carcinoma(IDC). Methods: 59 cases of DCIS paraffin embedded specimens (DCIS group), 32 cases of IDC paraffin embedded specimens (IDC group) in Hubei Medical Group of breast and thyroid surgery of Huangshi Central Hospital from January 2012 to August 2017 were selected, 20 cases of normal breast tissue samples during the same period were selected as control group. The expressions of MMP-7, VEGF and E-cad in each group were detected. The relationship between the positive expression rates of MMP-7, VEGF and E-cad and the clinicopathological features of patients with DCIS and IDC were analyzed. The correlation between MMP-7, VEGF and E-cad were analyzed by Pearson correlation. Results: The positive expression rates of MMP-7 and VEGF in DCIS group and IDC group were higher than those in the control group, the strong positive expression rate of E-cad was lower than that in the control group (P<0.05); there was no significant difference in the positive expression rate of MMP-7, VEGF and E-cad between DCIS group and IDC group (P>0.05). The positive rates of MMP-7, VEGF and E-cad were not related to the age of the patients and the size of the tumor (P>0.05). The positive expression rates of MMP-7 and VEGF in the patients with II-III stage, intermediate / low degree of differentiation and lymph node metastasis were higher than those of the patients with I stage, high differentiation degree and no lymph node metastasis (P<0.05). The positive rate of E-cad in patients with moderate / low differentiation and lymph node metastasis was lower than those of patients with high differentiation and no lymph node metastasis(P<0.05). Pearson correlation analysis showed that there was a positive correlation between MMP-7 and VEGF(r=0.362, P=0.038), MMP-7 and VEGF were not significantly correlated with E-cad (r=0.071,0.024,P=0.057, 0.089). Conclusion:: The expressions of MMP-7 and VEGF in DCIS group and IDC group are higher, but the expression of E-cad is lower, which is related to the clinical stage, degree of differentiation and lymph node metastasis of the patients. The development of breast cancer can be evaluated by examining the expressions of MMP-7, VEGF and E-cad.