汉黄芩苷通过调节抗氧化基因表达和机体代谢延长果蝇寿命

为探究汉黄芩苷是否具有抗衰老作用，本研究以果蝇为模型，考察汉黄芩苷对果蝇自然寿命的影响。采用RT-PCR和UPLC-MS/MS 代谢组学技术，探索汉黄芩苷发挥抗衰老作用的潜在机制。结果显示，0.02和0.5 mg/mL 汉黄芩苷均可整体延长果蝇寿命，并能够分别延长果蝇平均寿命5.64%和5.39%，延长最高寿命2.74%和5.12%；与30 d组相比，汉黄芩苷能够显著上调果蝇体内抗氧化酶基因SOD1、SOD2和CAT的表达水平，下调MTH的表达水平。果蝇代谢组学分析共找到17个潜在生物标志物，主要参与氨基酸代谢（D-谷氨酰胺和D-谷氨酸代谢，丙氨酸、天冬氨酸和谷氨酸代谢，精氨酸和脯氨酸代谢，缬氨酸、亮氨酸和异亮氨酸代谢）和能量代谢（氮代谢）。该结果表明，汉黄芩苷延缓衰老与上调抗氧化基因表达和调控不同代谢途径有关。

Wogonoside Extends the Lifespan of Drosophila melanogaster by Regulating the Expression of Antioxidant Genes and Body Metabolism

To investigate whether wogonoside has an anti-aging role, we studied its effect on the natural lifespan of Drosophila melanogaster. Metabonomics techniques, such as RT-PCR and UPLC-MS/MS, were used to explore the potential anti-aging effect of wogonoside. The results showed that 0.02 mg/mL and 0.5 mg/mL wogonoside could significantly prolong the overall longevity of fruit flies and improve the mean lifespan by 5.64% and 5.39%, and the maximum lifespan by 2.74% and 5.12%, respectively. Dietary supplement of wogonoside significantly up-regulated the expression of antioxidant genes SOD1, SOD2 and CAT, and down-regulated the expression of MTH in fruit flies, compared with the 30 d group. Seventeen potential biomarkers regulated by wogonoside treatment were found. The metabolic pathway analysis suggested that wogonoside antagonizes senescence mainly through modulating amino acid metabolism (D-glutamine and D-glutamate metabolism; alanine, aspartate and glutamate metabolism; arginine and proline metabolism; valine, leucine and isoleucine biosynthesis) and energy metabolism (nitrogen metabolism). The results suggest that the anti-aging role of wogonoside is associated with increasing the expression of antioxidant genes and regulating different metabolic pathways.