卵巢高/低级别浆液性癌的定量蛋白质组学比较研究

目的:探讨卵巢高级别浆液性癌和低级别浆液性癌的差异表达蛋白,为阐明卵巢癌发生机制及寻找诊断和预后标志物的提供线索。方法:收集卵巢癌新鲜组织标本冻存于液氮中,经病理学确诊为高级别浆液性癌和低级别浆液性癌,两种类型各收集15例。应用i TRAQ定量蛋白质组学技术筛选及鉴定高/低级别浆液性癌的差异表达蛋白,并进行生物信息学分析。结果:卵巢高级别和低级别浆液性癌组织的定量蛋白质组学比较研究鉴定出差异表达蛋白314个,其中与低级别浆液性癌组比较,高级别浆液性癌组上调蛋白有97种,下调蛋白有217种。GO分析显示这些差异蛋白在分子功能、生物学功能、细胞成分方面均具有一定分布特点。KEGG分析显示这些差异蛋白涉及复杂的信号通路。结论:高/低级别浆液性癌之间存在差异表达蛋白,这些蛋白涉及复杂的功能和信号通路可能在两型卵巢癌发生机制及肿瘤生物学行为差异中具有重要意义。

Quantitative Proteomic Analysis of Differentially Expressed Proteins in High/low Grade Serous Ovarian Cancer

ABSTRACT Objective: To investigate the differentially expressed proteins between ovarian high-grade serous carcinomas and low-grade serous carcinomas, and to provide information for the elucidation of the pathogenesis of ovarian cancer, and for the identifica- tion of diagnostic and prognostic markers. Methods: Fresh tissue specimens of ovarian cancer were collected and kept in liquid nitrogen, which were diagnosed as high-grade serous carcinoma and low-grade serous carcinoma by both of pathologists, and 15 cases were col- lected in each of the two types. Screening and identification of differentially expressed proteins in serous carcinomas by iTRAQ quantita- tive proteomics technique and bioinformatics analysis. Results: Quantitative proteomics of high grade and low grade ovarian serous carci- noma showed that 314 differentially expressed proteins were identified . Compared with low-grade serous carcinoma group, high-grade serous carcinoma group have 97 up-regulated proteins , 217 down-regulated proteins. GO analysis showed that these differential proteins were distributed in molecular function, biological function and cell composition. KEGG analysis showed that these differential proteins were involved in complex signaling pathways. Conclusion: There are differentially expressed proteins between high and low serous carci- nomas. These proteins are involved in complex functions and signaling pathways, which may play an important role in the pathogenesis of two type of ovarian cancer and biological differences in tumor behavior.