窒息新生儿血清miR-21、miR-210及miR-199a的表达及临床意义

目的:探讨窒息新生儿血清mi RNA-21、mi RNA-210及mi RNA-199a的表达及临床意义。方法:选取2015年9月1日到2017年7月31日我院新生儿科收治的窒息新生儿40例作为观察组,另选取同期在我院出生的健康新生儿40例作为对照组,根据阿氏(Apgar)评分将观察组的新生儿分为重度窒息组(12例)和轻度窒息组(28例)。检测所有新生儿血清中mi RNA-21、mi RNA-210及mi RNA-199a的表达水平,并比较观察组与对照组、重度窒息组与轻度窒息组血清中mi RNA-21、mi RNA-210及mi RNA-199a水平,并分析其相关性。结果:观察组的血清mi RNA-21、mi RNA-210水平高于对照组,mi RNA-199a水平低于对照组,差异有统计学意义(P0.05)。重度窒息组的血清mi RNA-21、mi RNA-210水平均高于轻度窒息组,mi RNA-199a水平低于轻度窒息组,差异有统计学意义(P0.05)。观察组血清mi RNA-21与mi RNA-210呈正相关(P0.05),血清mi RNA-21、mi RNA-210与mi RNA-199a水平呈负相关(P0.05)。结论:窒息新生儿血清中mi RNA-21、mi RNA-210呈现高表达,mi RNA-199a呈现低表达,且其表达水平与窒息严重程度相关。

The Expression and Clinical Significance of Serum miR-21, miR-210 and miR-199a in Asphyxiated Neonates

ABSTRACT Objective: To investigate the expression and clinical significance of serum miRNA-21, miRNA-210 and miRNA-199a in asphyxiated neonates. Methods: 40 asphyxiated neonates who were treated in Neonatal department of our hospital from September 1,2015 to July 31, 2017 were selected as observation group, another 40 healthy newborns born in our hospital during the same period were selected as control group, according to the score of Apgar, the newborns in the observation group were divided into severe asphyxia group (12 cases) and mild asphyxia group (28 cases). The expression levels of serum miRNA-21, miRNA-210 and miRNA-199a of all newborns were detected, the levels of serum miRNA-21, miRNA-210 and miRNA-199a in the observation group and the control group, severe asphyxia group and mild asphyxia group were compared, and analyzed their correlation. Results: The levels of serum miRNA-21 and miRNA-210 in the observation group were higher than those in the control group, and the level of serum miRNA-199a was lower than that in the control group, the differences were statistically significant (P<0.05). The levels of serum miRNA-21 and miRNA-210 in the severe asphyxia group were higher than those in the mild asphyxia group, and the level of serum miRNA-199a was lower than that in the mild asphyxia group, the differences were statistically significant (P<0.05). Serum miRNA-21 was positively correlated with miR- NA-210 in the observation group (P<0.05), and serum miRNA-21 and miRNA-210 were negatively correlated with miRNA-199a levels (P<0.05). Conclusion: The expressions of serum miRNA-21 and miRNA-210 in the asphyxia neonatorum is high, serum miRNA-199a is low, and the expression level is relate to the severity of asphyxia.