结直肠癌组织RSK4、p53蛋白的表达及临床病理意义

目的:探讨结直肠癌组织中P90核糖体S6激酶4(RSK4)蛋白、p53蛋白的表达及其临床病理意义。方法:选取我院病理科2014年1月~2016年5月既往收集的结直肠癌手术后标本70例及同期结直肠癌癌旁组织30例,采用免疫组化染色检测两组标本中RSK4蛋白、p53蛋白的表达情况,并分析其与结肠癌患者临床病理特征的相关性。结果:结直肠癌组织中RSK4蛋白、p53蛋白的阳性表达率分别为20.00%、55.71%,而癌旁组织中RSK4蛋白、p53蛋白阳性表达率分别为53.33%、10.00%,两组比较差异均具有统计学意义(P0.05)。Ⅰ期+Ⅱ期、高分化和中分化结直肠癌组织RSK4蛋白的阳性表达率显著高于Ⅲ期、低分化结直肠癌(P0.05);Ⅰ期+Ⅱ期、浸润深度(T1、T2)、未发生淋巴结转移的结直肠癌组织中p53蛋白阳性表达率显著的低于Ⅲ期、浸润深度(T3、T4)、发生淋巴结转移的结肠癌组织(P0.05)。结论:结直肠癌组织中RSK4蛋白表达下调、p53蛋白表达上调,二者可能与结直肠癌的发生和发展有关,并可能作为结肠癌诊断和预后评估的参考指标。

Expressions of RSK4 and p53 Protein in the Colorectal Cancer and the Clinicopathological Significances

ABSTRACT Objective: To investigate the protein expressions of P90 ribosomal S6 kinase 4 (RSK4) and p53 in the colon cancer and their clinicopathological significances. Methods: A total of 70 cases of colorectal cancer specimens and 30 cases of adjacent colorectal tissue specimens were collected in our hospital from January 2014 to May 2016, the expressions of RSK4 protein and p53 protein were detected using immunohistochemical staining, and the correlation of RSK4 and p53 protein expressions with the clinicopathological characteristics of colorectal cancer patients were analyzed. Results: The positive expression rates of RSK4 protein and p53 protein in the colorectal cancer were 20% and 55.71%, which were 53.33%, 10% in the adjacent tissues and significantly different from the colorectal cancer tissue(P<0.05); the positive expression rate of RSK4 protein in the colorectal cancer with phase I+II, high differentiation were significantly higher than those in stage III, poorly differentiated colorectal cancer(P<0.05); the positive expression rate of p53 in the colorectal cancer with phase I + II, the depth of invasion (T1, T2), no lymph node metastasis were significantly lower than those in stage III, the depth of invasion (T3, T4), lymph node metastasis of colon cancer(P<0.05). Conclusion: The expression of RSK4 protein was down regulated and the expression of p53 protein was up-regulated in the colorectal cancer tissues, which might be closely related to occurrence and development of colorectal cancer and be used as references for the diagnosis and prognostic prediction of colorectal cancer.