miR-21在疤痕形成中的作用及其机制研究

目的:探究mi R-21在疤痕形成中的作用及其相关机制。方法:选取2016年3月-2017年8月在本院就诊的20例皮肤有疤痕患者,采集患者的正常皮肤组织(正常组)、疤痕组织(疤痕组),进行成纤维细胞的分离培养,比较正常组、疤痕组mi R-21与Smad-7的m RNA表达水平,并在疤痕组织加入mi R-21抑制剂(抑制组),比较三组成纤维细胞增殖情况、蛋白表达及磷酸化水平。结果:正常组中Smad-7的m RNA表达水平显著高于疤痕组,而mi R-21的m RNA水平显著低于疤痕组,差异均有统计学意义(P0.05)。细胞培养48 h和72 h时,疤痕组成纤维细胞增殖水平明显高于正常组和抑制组,差异有统计学意义(P0.05),而抑制组和正常组成纤维细胞增殖水平比较差异无统计学意义(P0.05)。疤痕组成纤维细胞Smad-7蛋白表达低于正常组和抑制组,差异有统计学意义(P0.05),疤痕组成纤维细胞Smad-2、Smad-3蛋白表达及磷酸化水平均高于正常组和抑制组,差异有统计学意义(P0.05)。结论:疤痕组织中mi R-21通过负调控Smad-7的表达,从而引起Smad-2与Smad-3蛋白表达发生变化,使得疤痕成纤维细胞的增殖水平明显增高,最终促成疤痕的形成。

Study on the Role of miR-21 in Scar Formation and Its Mechanism

ABSTRACT Objective: To explore the role of miR-21 in scar formation and its related mechanisms. Methods: 20 cases of skin scar patients who were diagnosed in our hospital from March 2016 to August 2017 were selected,collected the normal skin tissue (normal group), scar tissue (scar group), the isolation and culture of fibroblasts, the mRNA expression level of miR-21 and Smad-7 in normal group and scar group were compared, MiR-21 inhibitor (inhibition group) was added to the scar tissue, the fibrous cell proliferation, pro- tein expression and phosphorylation level between the three groups were compared. Results: The mRNA expression level of Smad-7 in normal group was significantly higher than that in scar group, but the mRNA level of miR-21 was significantly lower than that of scar group, and the differences were statistically significant (P<0.05). When cell culture was 48 h and 72 h, the proliferation level of the fi- brous cell in scar group was significantly higher than that in normal group and inhibitory group, the difference was statistically significant (P<0.05), but there was no statistically significant difference between the inhibition group and normal group (P>0.05). The expression of Smad-7 protein of fibrous cell in scar group was lower than that of normal group and inhibitory group, the difference was statistically sig- nificant (P<0.05), the expression of Smad-2 and Smad-3 protein and phosphorylation level of of the fibrous cell in scar group were higher than those of normal group and inhibitory group, the differences were statistically significant (P<0.05). Conclusion: miR-21 in scar tissue through negative regulation of Smad-7 expression, resulting in changes of Smad-2 and Smad-3 protein expression, the proliferation of scar fibroblasts increased significantly, eventually contributing to the formation of scars.