蓝莓浓缩汁对小鼠疲劳的改善作用及机制初探

目的:对蓝莓浓缩汁进行抗疲劳功效评价,并对其所涉及的作用机制进行初步探究。方法:小鼠通过预筛选和强制游泳预处理后,以生理盐水为对照组,蓝莓浓缩汁高、低剂量和VC分别作为实验组。经给药及游泳处理后,观察各组小鼠的行为学特征,测定力竭游泳时间,检测血浆和脑组织匀浆中T-SOD活力、MDA含量、LDH活性及血浆中BUN含量的变化,并经HE染色察看小鼠组织病变状况。结果:与S对照组相比,高剂量蓝莓浓缩汁可明显改善疲劳小鼠的行为学特征,延长力竭游泳时间;在血浆和脑组织匀浆中,可增加T-SOD活性((136.92±10.39)%,(50.31±7.26)%),降低MDA含量((58.32±2.91)%,(50.89±2.91)%),提高LDH活性((72.79±19.11)%,(65.11±6.55)%),并减少血浆中BUN((46.92±3.36)%)的产生,(P0.01);还可防止细胞病变间隙的发生。低剂量蓝莓浓缩汁和VC处理组的改善效果不如高剂量组明显。结论:蓝莓浓缩汁有明显抗疲劳功效,所涉及的机制可能与其增强抗氧化酶活性,提高机体有氧代谢能力及改善机体物质代谢等有关。

The Effect and Mechanism of Concentrated Blueberry Juice on Anti-fatigue in Mice

ABSTRACT Objective: To evaluate the effect of the concentrated blueberry juice on anti-fatigue and survey the mechanism in- volved preliminarily. Methods: The mice were prescreened and forced swimming training. Then choosing the physiological saline as con- trol group, and choosing the high and low dose of concentrated blueberry juice and V _C as the experimental groups separately. After the processing of dosing and swimming training, the behavioral characteristics of each group mice was observed and the swimming exhaustion time was measured. The change of T-SOD activity, MDA content and LDH activity in the plasma and the brain tissue homogenate pro- tein was tested. The BUN content in the plasma was also tested. The method of HE stain was performed to observe the tissue pathological change of mice. Results: Compared with the control group of S, high dose of concentrated blueberry juice could improve the behavioral characteristics of fatigue mice obviously and prolong swimming exhaustion time. It could improve T-SOD activity ((136.92±10.39)%, (50.31±7.26)%), reduce the production of MDA ((58.32±2.91)%, (50.89±2.91)%) and improve LDH activity ((72.79±19.11)%, (65.11±6.55)%) of the plasma and the brain tissue homogenate. Besides it could reduce the production of BUN ((46.92±3.36)%) of the plasma, (P<0.01). It also could prevent the formation of cell clearance. Compared with the high dose concentrated blueberry juice, the improved effect of low dose concentrated blueberry juice and VC treatment group was not obvious. Conclusion: Concentrated blueberry juice has significant anti-fatigue effect. The mechanism may be involved with enhancing the antioxidant enzyme activity, improving the body''s aerobic metabolism capacity and improving the body''s metabolism, etc.