胃癌组织中微小RNA-375基因甲基化及其表达与临床病理特征的关系研究

目的:研究胃癌组织中微小RNA-375(miRNA-375)基因甲基化及其表达与临床病理特征的关系。方法:选择从2015年9月到2017年6月在我院接受手术治疗的胃癌患者60例纳入研究对象,将术中切除的癌组织标本60例作为观察组;另选同期在我院治疗的浅表性胃炎患者60例,将经内镜采集到的正常胃黏膜标本60例作为对照组。检测并对比两组患者miRNA-375基因甲基化及表达水平,分析胃癌患者的miRNA-375基因表达水平及甲基化与其病理特征的关系,并采用Spearman法分析miRNA-375基因表达水平、基因甲基化与其分化程度的相关性。结果:观察组miRNA-375基因的表达水平为(0.034±0.021),明显低于对照组的(0.187±0.104),差异有统计学意义(P0.05)。miRNA-375基因甲基化的阳性率为61.67%(37/60),明显高于对照组的20.00%(12/60),差异有统计学意义(P0.05)。低分化胃癌患者的miRNA-375基因表达水平明显低于中、高分化者,差异有统计学意义(P0.05)。低分化胃癌患者的miRNA-375甲基化阳性率明显高于中高分化者,差异有统计学意义(P0.05)。根据Spearman相关性分析发现,胃癌患者的miRNA-375基因表达水平与其分化程度呈正相关(P0.05),基因甲基化与其分化程度呈负相关(P0.05)。结论:胃癌组织中的miRNA-375基因的表达水平明显下降,而基因甲基化的比例明显上升,且二者均与肿瘤的分化程度有关,可考虑在临床上将其作为监测靶点,从而更好地评价患者的病情及预后。

Expression of miRNA-375 Gene Methylation in Gastric Carcinoma and Its Relationship with Clinicopathological Features

ABSTRACT Objective: To study the expression of miRNA-375 gene methylation in gastric carcinoma and its relationship with clin- icopathological features. Methods: Sixty patients with gastric cancer, who received surgical treatment in Shaanxi Provincial People''s Hos- pital during September 2015 to June 2017, were enrolled in the study; 60 cases of resected cancer tissues were taken as observation group. 60 cases of superficial gastritis treated in the hospital during the same period were selected, 60 cases of normal gastric mucosa collected by endoscopy were used as control group. The methylation and expression level of miRNA-375 gene in the two groups were detected and compared. The expression of miRNA-375 gene methylation in the patients with gastric carcinoma and its relationship with clinicopatho- logical features was analyzed. The expression level of miRNA-375 gene and the correlation between gene methylation and its degree of differentiation were analyzed by Spearman. Results: The expression level of miRNA-375 gene(0.034±0.021) of the observation group was significantly lower than that(0.187±0.104) of the control group, the difference was statistically significant (P<0.05). The positive rate(61.67%)(37/60) of miRNA-375 gene methylation was significantly higher than that(20.00%)(12/60) of the control group, the differ- ence was statistically significant(P<0.05). The expression level of miRNA-375 gene in the patients with poorly differentiated gastric can- cer was significantly lower than that in the patients with middle and high differentiated gastric cancer, the difference was statistically sig- nificant (P<0.05). The positive rate of miRNA-375 methylation in the patients with poorly differentiated gastric cancer was significantly higher than that in the patients with middle and high differentiation, the difference was statistically significant(P<0.05). Spearman correla- tion analysis showed that the expression level of miRNA-375 gene was positively correlated with the degree of differentiation in gastric cancer patients (P<0.05), gene methylation was negatively correlated with its degree of differentiation (P<0.05). Conclusion: The expres sion level of miRNA-375 gene in the gastric cancer tissue is obviously decreased, but the proportion of gene methylation is obviously in- creased, and the two indexes are related to the degree of tumor differentiation, which can be considered as a monitoring target in the clin- ic in order to better evaluate the patient''s condition and prognosis.