积雪草甙对乳腺癌MCF-7细胞凋亡及VEGF、bFGF蛋白表达水平的影响

目的:探讨积雪草甙对乳腺癌MCF-7细胞凋亡及VEGF、bFGF蛋白表达水平的影响。方法:选取人乳腺癌细胞MCF-7细胞系进行体外培养后,根据是否进行积雪草甙干预而分为两组,应用积雪草苷进行干预后,HE染色后用光学显微镜法观察细胞形态学变化,干预后的24h、48h以及72h时,应用TUNEL技术对对细胞凋亡情况进行检测,同时应用免疫荧光法检测血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的表达。结果:(1)与对照组相比较,积雪草甙干预的乳腺癌MCF-7细胞出现空泡、胞质外溢以及胞核皱缩等细胞凋亡现象,大量癌MCF-7细胞发生破碎死亡;(2)TUNEL技术法检测结果证实积雪草甙能够提高人乳腺癌MCF-7细胞凋亡率,与对照组比较差异具有统计学意义(P0.05),且呈时间依赖性;(3)积雪草甙干预的MCF-7细胞VEGF阳性表达和bFGF阳性表达显著低于对照组,差异具有统计学意义(P0.05),积雪草甙的抑制作用且呈时间依赖性。结论:积雪草甙不仅能够促进乳腺癌MCF-7细胞凋亡,而且能够降低VEGF和bFGF表达。

Effects of Asiaticoside on the Apoptosis and Expressions of VEGF and bFGF in MCF-7 Cells

ABSTRACT Objective: To investigate the effects of asiaticoside on apoptosis and expression level of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in MCF-7 cells during the treatment of the Mammary cancer. Methods: Human breast cancer MCF-7 cell lines were cultivated in vitro and then divided into control and treatment groups based on whether application of asiaticoside intervention. After the application of asiaticoside intervention, cell morphological changes were stained by HE staining method and observed by optical microscopy. TUNEL method was used to detect the cell apoptosis and immuno fluorescence method was used to detect the expression levels of VEGF and bFGF after the intervention 24 h, 48 h and 72 h by asiaticoside. Results: (1) Compared with control group, breast cancer MCF-7 cells presented vacuoles, cytoplasm spillover, nucleus shrinkage, cell broken and so on apopto- sis phenomenons in the treatment group. (2) The TUNEL assay confirmed the asiaticoside treatment could improve human breast cancer cell apoptosis rate, and the rate presented time-dependent relations. Besides, the rate was significantly higher than that in control group (P<0.05); (3) The positive expression of VEGF and bFGF in cells after asiaticoside intervention were time-dependent and significantly lower than that in the control group(P<0.05); Conclusion: The asiaticoside could not only significantly promote the apoptosis of breast cancer cells, but also could reduce the expression of VEGF and bFGF.