IL-17和VEGF在慢性鼻-鼻窦炎患者鼻息肉组织中的表达及相关性研究

目的:探讨慢性鼻-鼻窦炎患者鼻息肉组织中白介素-17(IL-17)、血管内皮生长因子(VEGF)的表达,并分析IL-17、VEGF表达水平的相关性。方法:以我院2015年1月~2017年12月期间收治的慢性鼻-鼻窦炎患者95例为研究对象,按患者有无鼻息肉分为伴鼻息肉(观察1组)49例和不伴鼻息肉(观察2组)46例。另选取同期在我院进行治疗的鼻中隔偏曲患者40例为对照组。所有患者均进行鼻内镜手术治疗,并在术中取其较窄侧的鼻甲黏膜作为检测标本,采用免疫组化SP法检测各组织标本中的IL-17、VEGF的表达水平,并分析IL-17、VEGF表达水平的相关性。结果:观察1组患者IL-17、VEGF的阳性表达率分别为93.88%(46/49)、85.71%(42/49),均高于观察2组患者IL-17、VEGF的阳性表达率[76.09%(35/46)、65.22%(30/46)]以及对照组患者IL-17、VEGF的阳性表达率[5.00%(2/40)、2.50%(1/40)],差异均具有统计学意义(P0.05)。观察1组患者IL-17、VEGF的表达水平分别为(38.92±5.34)个/LP、(33.21±4.87)个/LP,均高于观察2组患者IL-17、VEGF的表达水平[(28.19±4.56)个/LP、(21.28±4.03)个/LP]以及对照组患者IL-17、VEGF的表达水平[(9.31±2.76)个/LP、(7.19±1.95)个/LP],差异均具有统计学意义(P0.05)。经Spearman相关性分析结果显示,观察1组患者、观察2组患者中,IL-17与VEGF的表达水平呈正相关性(P0.05)。结论:慢性鼻-鼻窦炎患者鼻息肉组织中IL-17、VEGF的表达显著升高,且IL-17与VEGF表达水平呈明显的正相关性,表明IL-17、VEGF可能共同参与鼻息肉的发生与发展过程。

Expression and Correlation of IL-17 and VEGF in Nasal Polyps of Patients with Chronic Rhinosinusitis

ABSTRACT Objective: To investigate the expression of interleukin-17(IL-17) and vascular endothelial growth factor (VEGF) in nasal polyp tissue of patients with chronic rhinosinusitis, and to analyze the correlation between IL-17 and VEGF expression levels. Methods: 95 patients with chronic rhinosinusitis who were treated in our hospital from January 2015 to December 2017 were selected as the re- search subjects. According to whether the patients had nasal polyps, they were divided into nasal polyps (Observation group 1) with 49 cases and no nasal polyps (Observation group 2) with 46 cases. In addition, 40 patients with nasal septum deviation who were treated in our hospital over the same period were selected as control group. All patients were underwent endoscopic surgery,and the nasal concha mucous membrane at the narrower side of patients was taken as a test specimen during the operation. The expression levels of IL-17 and VEGF in all tissue specimens were detected by immunohistochemical SP method, and the correlation of IL-17 and VEGF expression lev- els was analyzed. Results: The positive rates of IL-17 and VEGF in Observation group 1 were 93.88% (46/49) and 85.71% (42/49), re- spectively, which were all higher than those of [76.09% (35/46), 65.22% (30/46)] in Observation group 2 and [5.00% (2/40) and 2.50% (1/40)] in the control group, the differences were statistically significant (P<0.05). The expression levels of IL-17 and VEGF in Observa- tion group 1 were (38.92±5.34)amount/LP and (33.21±4.87)amount/LP, respectively, which were all higher than those of [(28.19±4.56)amount/LP,(21.28±4.03)amount/LP] in Observation group 2 and [(9.31±2.76)amount/LP, (7.19±1.95)amount/LP] in the control group, the differences were statistically significant (P<0.05). The results of Spearman correlation analysis showed that there was a posi- tive correlation between the expression levels of IL-17 and VEGF of patients in Observation group 1 and Observation group 2 (P<0.05). Conclusion: The expression of IL-17 and VEGF in the nasal polyp tissue of patients with chronic rhinosinusitis is increased significantly, and the expression level of IL-17 is significantly positively correlated with VEGF expression, it is suggested that IL-17 and VEGF may participate in the occurrence and development of nasal polyps.