TRPC3对上皮性卵巢癌细胞株迁移和侵袭的影响

目的:探讨瞬时受体电位通道C3(TRPC3)对人卵巢癌细胞迁移、侵袭能力的影响。方法:采用蛋白免疫印迹法和实时荧光定量PCR法分别检测卵巢癌细胞株SKOV3、ES-2和HEY-T30中TRPC3蛋白和m RNA的表达水平。通过Transwell迁移实验(不含Matrigel胶的Transwell小室)和Transwell侵袭实验分别检测卵巢癌细胞株SKOV3、ES-2和HEY-T30的迁移、侵袭能力。结果:在SKOV3、ES-2和HEY-T30三种卵巢癌细胞株中,ES-2中TRPC3的蛋白和m RNA表达均显著高于其他两株(P0.05)。Transwell迁移实验和Transwell侵袭实验显示卵巢癌细胞株ES-2的迁移、侵袭能力均显著高于其他两种细胞株(P0.05)。结论:瞬时受体电位通道C3(TRPC3)在ES-2人卵巢癌细胞中高表达,并可能促进人卵巢癌细胞的迁移、侵袭。

Effect TRPC3 Act on the Migration and Invasion of Ovarian Cancer Cells

ABSTRACT Objective: To study the effect of the migration and invasion of the Transient receptor potential channel (TRPC3) on the Ovarian cancer cell. Methods: The expression levels of the TRPC3 in human ovarian carcinom Cell SKOV3, ES-2 and HEY-T30 were detected by western blot and fluorescent quantitative PCR. The Wound Healing assay an d T ransw ell assay were performed to analyze the ability of Migration an d invasion. Results: The expressions of TRPC3 in the ES-2 ovarian cancer cell lines were higher than the other two ovarian epithelial cancer cell lines by western blot and fluorescent quantitative PCR. The migration and invasion ability of the human ovarian carcinom ES-2 is hingher than the other two ovarian epithelial cancer cell line. Conclusion: The transient receptor potential chan- nel C3 may contributes to the migration and invasion of human ovarian cancer cells.