microRNA-151-5p在肾血管性高血压大鼠血管内皮细胞中的表达及意义

目的:研究microRNA-151-5p(miR-151-5p)在两肾一夹(2K1C)肾血管性高血压大鼠中的表达,并为miR-151-5p参与调节血管内皮细胞功能提供理论依据。方法:建立2K1C大鼠模型,获得胸主动脉血管内皮,采用荧光定量PCR技术检测血管内皮细胞中miR-151-5p的表达,通过数据库及生物信息学软件预测miR-151-5p的靶基因,并对靶基因进行GO富集和KEGG pathway分析。结果:与假手术组大鼠相比较,实验组大鼠胸主动脉血管内皮细胞miR-151-5p的表达量显著升高(P0.05)。GO分析显示miR-151-5p的靶基因参与蛋白加工水解、Notch受体加工等多种生物学功能(P0.01);KEGG pathway分析显示miR-151-5p的靶基因参与Notch信号通路、血管平滑肌收缩、代谢途径、细菌感染和RNA转运等信号通路。结论:2K1C大鼠血管内皮细胞中miR-151-5p表达升高,可能通过对其靶基因APH1A的调控参与血管内皮细胞功能调节。

Expression and Significance of MicroRNA-151-5p in the Vascular Endothelial Cells of Renovascular Hypertensive Rats

ABSTRACT Objective: To study the expression of microRNA-151-5p (miR-151-5p) in two kidney one clip (2K1C) renovascular hypertensive rats, and provide a theoretical guidance for miR-151-5p to participate in the regulation of vascular endothelial cell function. Methods: 2K1C rat model was established to obtain the endothelium of thoracic aorta. The expression of miR-151-5p in vascular endothelial cells was detected by fluorescent quantitative PCR. We also predicted target genes of miR-151-5p by database and bioinformatics software. Furthermore, we analyzed the function of target gene by KEGG and GO pathways. Results: Compared with the sham group, the expression of miR-151-5p in thoracic aorta endothelial cells in experimental group was significantly higher (P<0.05). In GO analysis, the target genes of miR-151-5p were enriched in many biological process,such as regulation of protein processing and regulation of Notch receptor processing(P<0.01). In KEGG pathway, the target genes of miR-151-5p were involved in Notch signaling pathway, vascular smooth muscle contraction, metabolic pathway, bacterial infection and RNA transport pathway. Conclusion: MiR-151-5p expression increased in 2K1C rats, it may regulate the function of vascular endothelial cell by its target gene APH1A.