Graphitized macroporous carbon microarray with hierarchical mesopores as host for the fabrication of electrochemical biosensor |
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Authors: | Xianbo Lu Yi Xiao Zhibin Lei Jiping Chen |
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Institution: | aDalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;bInstitute of Chemistry for Functionalized Materials, Faculty of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China |
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Abstract: | A novel graphitized ordered macroporous carbon (GMC, pore size 380 nm) with hierarchical mesopores (2–30 nm) and high graphitization degree was prepared by nickel-catalyzed graphitization of polystyrene arrays. The obtained GMC possessed high specific surface area, large pore volume, and good electrical conductivity, which was explored for the enzyme entrapment and biosensor fabrication by a facile method. With advantages of novel nanostructure and good electrical conductivity, direct electrochemistry of hemoglobin (a model protein) was observed on the GMC-based biocomposite with a formal potential of −0.36 V (vs. Ag/AgCl) and an apparent heterogeneous electron transfer rate constant (ks) of 1.2 s−1 in pH 7.0 buffer. Comparative studies revealed that GMC offered significant advantages over carbon nanotubes (CNTs) in facilitating direct electron transfer of entrapped Hb. The fabricated biosensor exhibited good sensitivity (101.6 mA cm−2 M−1) and reproducibility, wide linear range (1–267 μM), low detection limit (0.1 μM), and good long-term stability for H2O2 detection. GMC proved to be a promising matrix for enzyme entrapment and biosensor fabrication, and may find wide potential applications in biomedical detection and environmental analyses. |
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Keywords: | Biosensor Graphitized ordered macroporous carbon Direct electron transfer Hemoglobin Hydrogen peroxide |
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