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Humanization of fibroblast growth factor 1 single‐chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells |
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| Authors: | Xiao‐Xiao He Shuang Du Shi‐Qian Gao Jing‐Ying Chen Ran‐Juan Cao Zhen‐Kai Xing Alia Rizvi Syeda Kazim Hua‐Li Yu Qing‐Chuan Zheng Xiao‐Juan Zhu |
| Institution: | 1. Key Laboratory of Molecular Epigenetics of Ministry of Education, Institute of Genetics and Cytology, Northeast Normal University, Changchun, China;2. Department of Hand Surgery, China‐Japan Union Hospital of Jilin University, Changchun, China;3. Laboratory of Theoretical and Computational Chemistry, Institute of Theoretical Chemistry, Jilin University, Changchun, China |
| Abstract: | Single‐chain variable fragment (scFv) antibodies are the smallest immunoglobulins with high antigen‐binding affinity. We have previously reported that fibroblast growth factor 1 played pivotal roles in cancer development and generated a mouse scFv (mscFv1C9) could effectively prohibit cancer cell proliferation in vitro and in vivo. Here, we further humanized this scFv (hscFv1C9) using a structure‐guided complementarity determining region grafting strategy. The purified hscFv1C9 maintained similar antigen‐binding affinity and specificity as mscFv1C9, and it was capable of inhibiting growth of different tumours in vitro and in vivo. These data strongly suggested that hscFv1C9 has antitumour potentials. |
| Keywords: | antibody humanization cancer treatment fibroblast growth factor 1 monoclonal antibody single‐chain variable fragment |