Identification and characterization of bacterial-binding property in the type III repeat domain of fibronectin |
| |
Authors: | Ito Hiro-O Soutome Sakiko Nokihara Kiyoshi Inoue Masakazu |
| |
Affiliation: | Department of Preventive Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan. dearkids@dentb.hal.kagoshima-u.ac.jp |
| |
Abstract: | To characterize fibronectin binding with Granulicatella adiacens, a causative agent of infective endocarditis, monoclonal antibodies were generated against human fibronectin and selected for their capacity to inhibit the fibronectin binding of the organism. Thermolysin and lysyl-endopeptidase digests of fibronectin were characterized by Western blot. The epitope of inhibitory monoclonal antibody was found in the central portion of fibronectin known as the cell-binding domain, and not in the N-terminal portion known to be the binding region of most microbial species, e.g., Staphylococcus aureus and Streptococcus pyogenes. While these two species could bind to both the N-terminal and central portion, Escherichia coli and G. adiacens bind only to the latter. Excess amounts of free fibronectin in the solution inhibited the bacterial adherence to the N-terminal fibronectin fragment, but not to the central region, thereby suggesting the central region plays a significant role for in vivo bacterial colonization in the presence of high concentrations of soluble fibronectin. |
| |
Keywords: | Bacterial adherence Fibronectin Monoclonal antibodies Bacterial infections Infective endocarditis Streptococcus pyogenes Staphylococcus aureus Granulicatella adiacens |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|