Effect of high nitrate concentrations on growth and nitrate uptake by free-living and immobilizedChlorella vulgaris cells |
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Authors: | J Jeanfils M-F Canisius N Burlion |
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Institution: | (1) ISSEP, Rue du Chéra 200, B-4000 Liège, Belgium;(2) Université de Mons-Hainaut, Faculté de Médecine, Rue du Parc 24, B-7000 Mons, Belgium |
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Abstract: | Growth and nitrate uptake were studied on free-living and immobilizedChlorella vulgaris cells cultivated in medium containing different nitrate concentrations. First, the effect of nitrate concentrations on growth indicated that cells can live in the presence of high concentrations as high as 97 mM. Although no lethal effect on cells was observed such concentration a slow down in growth and a decrease in biomass produced was observed. The rate of nitrate uptake increased with the nitrate concentration in the medium. The maximum uptake rate was reached in first days of culture in both free-living and immobilized cells. The rate dropped more rapidly for cells growing in 2 mM nitrate than for cells growing in higher nitrate concentration. The maximum rate was very much the same for free-living and immobilized and was within the order of 0.45 to 0.57 g NO3 h–1 10–6 cells. Immobilization modified the changes of nitrate uptake rate for concentration higher than 2 mM. |
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Keywords: | nitrate uptake growth immobilized cells alginate bead Chlorella vulgaris |
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