Nitrogen metabolism of asparagine and glutamate in Vero cells studied by 1H/15N NMR spectroscopy |
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Authors: | Haiyan Huang Yihua Yu Xiaoping Yi Yuanxing Zhang |
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Affiliation: | (1) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China;(2) Shanghai Key Laboratory of Functional Magnetic Resonance Imaging and Department of Physics, East China Normal University, Shanghai, 200062, China |
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Abstract: | Glutamine-free culture of Vero cells has previously been shown to cause higher cell yield and lower ammonia accumulation than that in glutamine-containing culture. Nitrogen metabolism of asparagine and glutamate as glutamine replacer was studied here using nuclear magnetic resonance (NMR) spectroscopy. 15N-labelled glutamate or asparagine was added and their incorporation into nitrogenous metabolites was monitored by heteronuclear multiple bond coherence (HMBC) NMR spectroscopy. In cells incubated with l-[15N]glutamate, the 15N label was subsequently found in a number of metabolites including alanine, aspartate, proline, and an unidentified compound. No detectable signal occurred, indicating that glutamate was utilized by transamination rather than by oxidative deamination. In cells incubated with l-[2-15N]asparagine, the 15N label was subsequently found in aspartate, the amine group of glutamate/glutamine, and in two unidentified compounds. Incubation of cells with l-[4-15N]asparagine showed that the amide nitrogen of asparagine was predominantly transferred to glutamine amide. There was no detectable production of , showing that most of the asparagine amide was transaminated by asparagine synthetase rather than deaminated by asparaginase. Comparing with a glutamine-containing culture, the activities of phosphate-activated glutaminase (PAG), glutamate dehydrogenase (GDH) and alanine aminotransferase (ALT) decreased significantly and the activity of aspartate aminotransferase (AST) decreased slightly. |
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Keywords: | Asparagine Glutamate Glutamine-free medium 1H/15N HMBC NMR Nitrogen metabolism Vero cell |
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