铜绿假单胞菌泳动能力相关新基因的筛选及鉴定

从Mu转座突变子文库中经过表型筛选,得到12株泳动(Swimming motility)能力缺陷的突变子,经Mu转座子插入位点的确认、基因克隆及测序分析发现其中10个突变子中Mu转座子分别插入到10个不同的与鞭毛运动和功能相关的基因中,2个突变子中Mu转座子插入到功能未知的新基因(PA2950和PA5022)中,电镜观察结果表明这2个突变株均具有完整的鞭毛,初步推测这2个基因可能是参与鞭毛泳动的能量代谢、趋化作用或信息传递的新基因。

Identification of two new genes involved in swimming motility of Pseudomonas aeruginosa

Pseudomonas aeruginosa,a typical opportunistic pathogen,has a single polar flagella which provides swimming motility and virulence.Twelve of insertional mutants with Mu transposition complexes into genomic DNA of Pseudomonas aeruginosa were screened on the changes of swimming motility.After gene cloning and DNA sequencing of those mutants,ten of them were illustrated that defective mutations on swimming motility were caused by insertions of Mu transposon into 10 of different functional genes which may involved in biosynthesis of flagella and gene regulations,but another two mutants were resulted from the insertion of two new genes(PA2950,PA5022) with function unknown.Observation of these two mutants with an integrity flagellum under electron microscope showed that two new genes were not involved in construction of flagella,but probably in energy metabolism and gene regulation of swimming motility.