Isolation and characterization of polymorphic microsatellite loci in Achnatherum sibiricum (Poaceae) |
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| Authors: | Na Chen Yang Yang Chun-Jie Li Zhi-Biao Nan |
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| Affiliation: | (1) Key Laboratory of Grassland Agro-ecosystem, Ministry of Agriculture, College of Pastoral Agriculture Science and Technology, Lanzhou University, Gansu Grassland Ecological Research Institute, P.O. Box 61, Lanzhou, 730020, China |
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| Abstract: | Achnatherum sibiricum is a threaten and toxic perennial bunchgrass mainly in the north of China. We developed 11 polymorphic microsatellite loci from A. sibiricum by combining biotin capture method. After validating and scoring, these loci were polymorphic in a test population with the range of alleles from four to 13 per locus. The observed and expected heterozygosities ranged from 0.1649 to 0.5624 and from 0.3071 to 0.8826, respectively. All 11 microsatellite markers were in Hardy–Weinberg equilibrium. These polymorphic microsatellites will be useful for genetic diversity analysis and population structure construction for A. sibiricum. |
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| Keywords: | Achnatherum sibiricum Genetic diversity Microsatellites Conservation |
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