Curing effect of clorobiocin on Escherichia coli plasmids |
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Authors: | Karel ejka, Inge Holubová Josef Hubá ek |
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Affiliation: | (1) Department of Molecular Biology and Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4-Kr, Czechoslovakia |
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Abstract: | Summary Clorobiocin, an inhibitor of the gyrB subunit of DNA gyrase, was used for the curing of some Escherichia coli plasmids. Of the plasmids studied, ampicillin resistant R28K and a miniplasmid derived from R1 drd-19 were effectively eliminated. We also succeeded in eliminating the ColA factor from E. coli strain B834 (pBS103), which was resistant to the effect of currently used curing agents. Although a derivative of ColE1-pBR322 was effectively cured by clorobiocin, the ColE1-plasmid was resistant to its effect. The ColV plasmid determining virulence was effectively eliminated. |
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