A yeast gene coding for a putative protein kinase homologous to cdc25 suppressing protein kinase |
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Authors: | S Ohno M Aoshima S Matsumoto I Yahara K Suzuki |
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Institution: | Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan. |
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Abstract: | A yeast gene termed YKR coding for a putative protein kinase was isolated by using the cloned cDNA for rabbit protein kinase C as a hybridization probe. The encoded protein (YKR), composed of 380 amino acid residues, shows extensive sequence homology to serine/threonine-specific protein kinases from various species in the approx. 320 C-terminal amino acid residues, strongly suggesting that YKR is endowed with a protein kinase activity. The observed homologies to the cdc25 suppressing protein kinase from yeast, the catalytic subunit of mammalian cAMP-dependent protein kinase, and mammalian protein kinase C were 76, 48 and 37%, respectively. Gene replacement experiments showed that YKR itself is not essential for cell proliferation. |
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