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Flower color modifications of Torenia hybrida by cosuppression of anthocyanin biosynthesis genes
Authors:Suzuki  Ken-ichi  Xue  Hui-min  Tanaka  Yoshikazu  Fukui  Yuko  Fukuchi-Mizutani  Masako  Murakami  Yasuyuki  Katsumoto  Yukihisa  Tsuda  Shinzo  Kusumi  Takaaki
Institution:(1) Institute for Fundamental Research, Research Center, Suntory Limited, Osaka , 618-8503, Japan
Abstract:White and blue/white varieties of Torenia hybrida were successfully obtained from the blue variety cv. Summerwave (SWB) by cosuppressing expression of two of the genes involved in anthocyanin biosynthesis; chalcone synthase (CHS) and dihydroflavonol 4-reductase (DFR). Such molecular breeding is the only precise and efficient way to create flower color variation in SWB due to its male and female sterility. Flower color and the degree of suppression varied between transgenic lines, and anthocyanin biosynthesis was more consistently suppressed in the dorsal petal lobes, ventral petal lobes and corolla tube than lateral petal lobes. A pink variety was obtained by cosuppressing the flavonoid 3prime,5prime-hydroxylase (F3prime5primeH) gene. Yellow torenia was obtained from T-33, an in-house cultivar that contained both carotenoids and anthocyanins, by cosuppression of CHS or DFR genes.
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