Evidence for the presence of two D-amino acid oxidases inPseudomonas aeruginosa PAO |
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Authors: | T Herbert Manoharan Kunthala Jayaraman |
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Institution: | 1. Department of Molecular Biology, School of Biological Sciences, Madurai University, 625 021, Madurai
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Abstract: | By the isolation of mutants that were unable to grow on L-hydroxyproline or DL-valine, it has been possible to demonstrate the presence of two different types of D-amino acid oxidase activities inPseudomonas aeruginosa PAO. One was the D-amino acid dehydrogenase, probably involved in the oxidation of a number of D-amino acids such as D-alanine, D-phenylalanine and D-valine. The other was the inducible oxidase, specific to the oxidation of allohydroxy-D-proline formed from L-hydroxyproline during its oxidation. Thus, it has been possible to delink the involvement of the general D-amino acid dehydrogenase in the oxidative breakdown of allohydroxy-Dsproline. |
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