Growth of and trehalase activity in the thermophilic fungusThermomyces lanuginosus |
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Authors: | A R S Prasad Ramesh Maheshwari |
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Institution: | 1. Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore
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Abstract: | The thermophilic fungus,Thermomyces lanuginosus, was grown in a glucose-asparagine liquid medium. Optimal mycelial growth occurred at 50°C. The conditions for sporulation were different from those required for vegetative growth. the former being favoured by lower nitrogen level and temperature. Trehalase (α, α-glu coside-l-glucohydrolase, EC 3.2.1.28) was one of the most active glycosidases at 50°C. Non-sporulating mycelium had higher levels of this enzyme than the sporulating mycelium. Trehalase was synthesized constitutively and its activity appears to be controlled by catabolite repression. |
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