N-cadherin is a useful marker for the progenitor of cardiomyocytes differentiated from mouse ES cells in serum-free condition |
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Authors: | Honda Masahiko Kurisaki Akira Ohnuma Kiyoshi Okochi Hitoshi Hamazaki Tatsuo S Asashima Makoto |
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Institution: | Department of Biological Sciences, Graduate School of Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. |
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Abstract: | Cardiomyocytes are known to differentiate spontaneously from embryonic stem (ES) cells when they formed aggregates, so called "embryoid bodies", in the presence of serum. In this study, we explored the induction of cardiomyocytes from mouse ES cells in chemically defined serum-free medium by using a mesoderm-inducing factor, BMP4. Comparing the different inductive methods, we found a candidate cell surface marker, N-cadherin, for cardiomyocyte progenitors from ES cells. N-cadherin-positive cells highly expressed cardiogenic markers, Nkx2.5, Tbx5, and Isl1, and showed a high differentiation rate into cardiomyocyte lineage. These results indicate that N-cadherin can be a useful cell surface marker for the progenitors of cardiomyocyte differentiated from ES cells in the serum-free culture. |
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Keywords: | Cardiomyocyte N-cadherin Flk1 Serum free Embryonic stem cell Cell surface marker Mesodermal induction Progenitor Brachyury Nkx2 5 |
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