An improved method for the detection of Dcm methylation in DNA molecules |
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| Authors: | M Reuter C D Pein V Butkus D H Krüger |
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| Institution: | Institute of Virology, Humboldt-University, Berlin, G.D.R. |
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| Abstract: | The intrinsic insensitivity of EcoRII recognition sites in RF DNAs of phage M13 and vector M13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. Since Dcm- DNA but not Dcm(+)-methylated DNA becomes susceptible under these conditions, this procedure constitutes an improvement of the Dcm methylation assay. |
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