Properties of spherical calcium pectate and alginate gels and their use in diffusion chromatography,solids separations and immobilization of enzymes and cells |
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Authors: | P Gemeiner L' Kurillová A Malovíková D Tóth D Toma?ovi?ová |
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Institution: | (1) Institute of Chemistry, Centre for Chemical Research, Slovak Academy of Sciences, 842 38 Bratislava, Czechoslovakia;(2) Institute of Experimental Biology and Ecology, Centre of Biological-ecological Sciences, Slovak Academy of Sciences, 814 34 Bratislava |
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Abstract: | Water-soluble acidic polysaccharides—deesterified pectins and carboxy-derivatives of starch—precipitated with calcium ions
were tested as precursors of spherical calcium gels. Pectates prepared from apple or citrus pectin, similarly to alginates,
are compounds forming spherical calcium gels stable in aqueous medium which have a relatively highly reproducible mass, particle
size, water content, shape, mechanical strength and shearing. Both the liquid-solid partition of low- and high-molar-mass
solutes and its kinetics proved to be reasonable features. Distribution of pore size in the above materials was estimated.
Detailed pictures of surface and of the interior of calcium beads in the scanning electron microscope are presented. The possible
use of calcium beads as enzyme carriers, as affinity matrixes and entrapment materials for diffusion chromatography, solids
separations and bioindication of a specific water pollution was evaluated. Calcium alginate beads were always used as reference
material. |
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