| 灰黄霉素高产菌F208发酵过程蛋白质表达差异的研究 |
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| 引用本文： | 李晶,柯崇榕,高媛媛,黄建忠. 灰黄霉素高产菌F208发酵过程蛋白质表达差异的研究[J]. 中国微生态学杂志, 2011, 23(11): 986-988,990 |
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| 作者姓名： | 李晶 柯崇榕 高媛媛 黄建忠 |
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| 作者单位： | 1. 福建卫生职业技术学院医学技术系,福建福州,350101
2. 福建师范大学工业微生物教育部工程研究中心,生命科学学院,福建省现代发酵技术工程研究中心,福建福州 350108 |
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| 摘 要： | 目的 为探寻灰黄霉素生物合成过程中的关键酶,以蛋白质组学技术手段分析灰黄霉素高产菌F208发酵过程蛋白质表达差异.方法 通过双向电泳联用质谱技术对F208发酵过程蛋白质组图谱进行比较分析.结果 研究发现灰黄霉素产生期( 192 h)F208表达的蛋白质与产生前期(72 h)有较大差异,并鉴定出在灰黄霉素产生高峰期蛋白表达量明显增加的两个特异点是丝氨酸羟甲基转移酶和S-腺苷甲硫氨酸合成酶.结论 成功建立灰黄青霉菌丝体总蛋白双向电泳技术体系.并鉴定出两个蛋白特异点,极可能与灰黄霉素生物合成有关.
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| 关 键 词： | 灰黄青霉 灰黄霉素 蛋白质组 双向电泳 质谱 |
Difference of protein expressions of highly griseofulvin-producing Penicillum griseofulrum F208 in fermentation process |
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| LI Jing , KE Chong-rong , GAO Yuan-yuan , HUANG Jian-zhong. Difference of protein expressions of highly griseofulvin-producing Penicillum griseofulrum F208 in fermentation process[J]. Chinese Journal of Microecology, 2011, 23(11): 986-988,990 |
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| Authors: | LI Jing KE Chong-rong GAO Yuan-yuan HUANG Jian-zhong |
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| Affiliation: | LI Jing1,KE Chong-rong2,GAO Yuan-yuan2,HUANG Jian-zhong2 (1.Department of Medical Technology,Fujian Health College,Fuzhou 350101,China,2.Engineering Research Center of Industrial Microbiology,Ministry of Education,Fujian Normal University College of Life Sciences,Engineering Research Center of Fujian Modem Fermentation Technology,Fuzhou 350108,China) |
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| Abstract: | Objective Difference of protein expressions of highly griseofulvin-producing Penicillum griseofulvum F208 during fermentation was confirmed by proteomics techniques in order to explore the key enzymes in the course of biosynthesis of griseofulvin. Method The techniques of two-dimensional gel electrophoresis and mass spectrometry were used to analyze the differential expression of proteins from P.griseofulvum F208 during the course of fermentation. Result The expressed proteins had been found to differ from ... |
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| Keywords: | Penicillium griseofulvum Griseofulvin Proteomics Two-dimensional electrophoresis Mass-spectrum |
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