| 双歧杆菌番茄培养基的初步研究 |
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| 引用本文： | 王春耀,张德纯,朱辉,李金玲.双歧杆菌番茄培养基的初步研究[J].中国微生态学杂志,2011,23(11):991-993. |
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| 作者姓名： | 王春耀 张德纯 朱辉 李金玲 |
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| 作者单位： | 重庆医科大学分子医学与肿瘤研究中心;病原生物学教研室,重庆400016 |
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| 摘 要： | 目的 研制双歧杆菌番茄培养基,为后期双歧杆菌发酵剂的研究奠定基础.方法 以番茄汁为基础,采用单次单因子法与Plackett-Burman( P-B)设计法相结合,对BL培养基进行优化.结果 将BL培养基的16种成分筛选为7种,即葡萄糖、多胨、胰胨、异麦芽寡糖、可溶性淀粉、磷酸二氢钾和番茄汁,为获得产菌量高、成本 低、制作工艺简单的双歧杆菌番茄培养基奠定了基础.结论 将传统的非统计法和统计法相结合,既可以降低生产成本,又能在制作过程中节约劳动力.
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| 关 键 词： | 双歧杆菌 番茄培养基 单次单因子法 Plackett-Burman设计法 |
Optimization of tomato medium for Bifidobacterium culture |
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| WANG Chun-yao
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ZHANG De-chun
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ZHU Hui
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LI Jin-ling.Optimization of tomato medium for Bifidobacterium culture[J].Chinese Journal of Microecology,2011,23(11):991-993. |
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| Authors: | WANG Chun-yao ZHANG De-chun ZHU Hui LI Jin-ling |
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| Institution: | WANG Chun-yao,ZHANG De-chun,ZHU Hui,LI Jin-ling (Department of Pathogenic Biology,Molecular Medicine & Tumor Research Center,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To create a culture medium using tomato as main material for the culture of Bifidobacterium. Method BL medium was optimized by one-factor-at-a-time method and Plackett-Burman Design. Result The 16 ingredients of BL culture medium were reduced to 7,including glucose,polypeptone,tryptone,IMO,soluble starch,potassium dihydrogen phosphate and tomato juice. Conclusion The resulted tomato medium can not only reduce the production costs,but also save labor during the production process. |
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| Keywords: | Bifidobacteriun Tomato culture medium One factor at a time Plackett-Burman design |
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