Integrated siRNA design based on surveying of features associated with high RNAi effectiveness |
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Authors: | Wuming Gong Yongliang Ren Qiqi Xu Yejun Wang Dong Lin Haiyan Zhou Tongbin Li |
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Institution: | (1) Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA |
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Abstract: | Background Short interfering RNAs have allowed the development of clean and easily regulated methods for disruption of gene expression.
However, while these methods continue to grow in popularity, designing effective siRNA experiments can be challenging. The
various existing siRNA design guidelines suffer from two problems: they differ considerably from each other, and they produce
high levels of false-positive predictions when tested on data of independent origins. |
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Keywords: | |
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