| 光敏生物素标记法制备HPV DNA探针 |
| |
| 引用本文： | 张晓燕,隋丽华.光敏生物素标记法制备HPV DNA探针[J].生物技术,1993,3(4):44-46. |
| |
| 作者姓名： | 张晓燕 隋丽华 |
| |
| 作者单位： | 哈尔滨医科大学微生物教研室 哈尔滨
(张晓燕，谷鸿喜，郭彩玲，李绍贤)，哈尔滨医科大学附属三院妇科 哈尔滨
(隋丽华，娄阁)，哈尔滨医科大学附属三院妇科 哈尔滨(关光伟) |
| |
| 摘 要： | 本文将PBR322与HPV重组质粒DNA转化大肠杆菌HB101株,经氨苄青霉素—四环素平板筛选转化成功的菌落,将其扩增、提取质粒DNA,纯化后用限制性内切酶酶切,琼脂糖凝胶电泳区分不同大小的限制性内切片段,并用电洗脱法回收7.9Kb的HPV DNA片段。在高能卤素灯光照下标记光敏生物素制备HPV DNA探针,并检测其特异性和敏感性。结果表明:此探针具有较高敏感性,可达2.5pg;并且具有较强的特异性。
|
| 关 键 词： | 人乳头瘤病毒 光敏生物素 DNA探针 |
The Preparation of Photobiotic Labelled DNA Probe of Human Papillomaviruses (HPV) |
| |
| Zhang Xiaoyan et al.The Preparation of Photobiotic Labelled DNA Probe of Human Papillomaviruses (HPV)[J].Biotechnology,1993,3(4):44-46. |
| |
| Authors: | Zhang Xiaoyan |
| |
| Institution: | Zhang Xiaoyan et al
Department of microbiology,Harbin Medical Univ.,Harbin |
| |
| Abstract: | The recombinant plasmid of HPV with PBR322 was transformated and am-plificated in E.coli HB101, and the Plasinids were isolated and Purified. After the digestion with Restriction eiidoruiclease, the 7.9kb fragments of HPV DNA were collected with electroelution method and then labelled with photobiotic as the HPV DNA probe. The results indicated that the probe had a higher sensitivity and specificity .As small as 2.5 pg of HPV DNA could be detected with this probe |
| |
| Keywords: | Human papillomaviruses Photobiotic labelled PNA probe Dot blot |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
