Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae |
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| Authors: | Van Dellen Katrina L Bulik Dorota A Specht Charles A Robbins Phillips W Samuelson John C |
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| Institution: | Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany St., Evans 426, Boston, Massachusetts 02118, USA. |
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| Abstract: | Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p. |
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