Expression of modified oxidase of D-aminoacids of <Emphasis Type="Italic">Trigonopsis variabilis</Emphasis> in methylotrophic yeasts <Emphasis Type="Italic">Pichia pastoris</Emphasis> |
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Authors: | V A Redo E K Novikova M A Eldarov |
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Institution: | 1.Bioengineering Center,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | Effective recombinant strains Pichia pastoris that produce functionally active hybrid of Trigonopsis variabilis D-aminoacids bond with chitin-connecting domain of chitinase A1 of Bacillus circulans (DAOcbd) were obtained. The dependence of DAOcbd production levels from production of the number of copies of “expression
cassette” integrated in the AOX1 locus of recombinant strains was studied. It was indicated that synthesized DAOcbd may be
easily purified and immobilized on chitin sorbents and possessed high specific activity. Produced strains and methods of their
cultivation and DAOcbd extraction may be used for development of technologies of obtaining of biocatalyzers in technological
processes of obtaining of 7-aminocepha-losporane acid. |
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