A high-speed,quasi-isocratic high-pressure liquid chromatography system for protein sequence analysis |
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| Authors: | Clyde Zalut HWilliam Harris |
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| Institution: | 1. Divisions of Immunology and Hematology/Oncology, Department of Medicine, Children''s Hospital Medical Center, Harvard Medical School, Boston, MA 02115, U.S.A.;2. Departments of Pediatrics and Biological Chemistry, Harvard Medical School, Boston, MA 02115, U.S.A. |
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| Abstract: | A simple, of less than 20 min duration, quasi-isocratic high-pressure liquid chromatographic system designed for effective analysis of phenylthiohydantoin-amino acids derived from Edman sequencing is described. A second 30-min quasi-isocratic system is presented which may be used when sequenced samples contain extraneous 254 nm-absorbing material. Conditions affecting chromatogram reproducibility are also examined. |
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| Keywords: | high-pressure liquid chromatography protein sequencing phenylthiohydantoin-amino acids |
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