Gravity sedimentation analysis of human blood leukocytes |
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| Authors: | N Catsimpoolas EM Skrabut AL Griffith CR Valeri |
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| Institution: | 1. Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;2. Naval Blood Research Laboratory, Boston University Medical Center, Boston, MA 02118, U.S.A. |
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| Abstract: | A new method of sedimentation analysis of human blood leukocytes is described. Platelets, lymphocytes, monocytes, and polymorphonuclear cells isolated from normal human peripheral blood have been analyzed alone and in mixture by gravity sedimentation employing a computerized scanning instrument. All four classes could be clearly resolved from each other exhibiting sedimentation velocities of 0.6 ± 0.00, 1.04 ± 0.11, 1.27 ± 0.15 and 1.89 ± 0.21 · 10?4 cm/s, respectively, at 37°C in a 2.5–6.25% Ficoll gradient in Medium 199. Less than 106 cells can be used for analysis. Possible applications of the method are discussed. |
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| Keywords: | gravity sedimentation human leukocytes cell separation |
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