Laboratoire de Physiologie végétale et Biochimie, Université de Neuchâtel, 20 rue de Chantemerle, 2000 Neuchâtel, Switzerland
Abstract:
A simple method is proposed for the analysis of the distribution and changes in membrane lipids subjected to different treatments (lipolytic, aging, etc.). This technique involves only one-thin layer chromatographic step followed by a scanning of the photographic negative of the charred thin layer. This method is time saving, inexpensive and does not require the technical skill usually demanded in lipidology. The precision of this method is compared with that obtained with the classical TLC-GLC method: its variability is roughly twice that of the TLC-GC method.