Physiology of the Rhizobium-legume association: I—The presence of bacterial DNA within the plant root |
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Authors: | A. A. Lepidi M. P. Nuti G. Bernacchi Rachele Neglia |
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Affiliation: | (1) Istituto di Microbiologia agraria e tecnica dell'Università di Pisa-Centro di Studio per la Microbiologia del Suolo del C.N.R., 56100 Pisa, (Italy) |
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Abstract: | Summary Rhizobium deoxyribonucleic acid has been detected within Vicia faba root cells by in situ hybridization and autoradiography after exposure of root apexes to Rhizobium viable cells. Reannealed regions are localized into the cortex cells; the presence of bacterial DNA is specific for the root tissue; labelled regions were not detectable within apexes exposed to non-nodulating strains or to bacteria other than Rhizobium; Rhizobium DNA was not detectable in tissues of plants other than its leguminous host. re]19750313 |
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