Laser Raman spectra of glucagon |
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Authors: | T C Jao G Marquez |
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Institution: | Department of Chemistry, University of Puerto Rico Mayaguez, Puerto Rico |
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Abstract: | Laser Raman spectroscopy study indicates that in concentrated fresh acidic solution (30 mg/ml), glucagon remains predominantly α-helix and not random-coil. The splitting of the amide III band into three components in the crystal at 1262, 1275, and 1295 cm?1 is due to the α-conformation as expected. The presence of a small fraction of β-conformation is demonstrated by the appearance of the weak band at 1230 cm?1 in the fresh solution. This study also established the frequencies of amide III′ bands for the α- and β-conformations of glucagon: 957 and 988 cm?1 for α and β forms, respectively. The conformations of acidic and basic glucagon solutions are apparently different. |
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