Restriction fragment length polymorphism of the human T cell receptor alpha gene |
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| Authors: | Edward J. Ball Lori Dombrausky Marie Hoover J. Donald Capra Peter Stastny |
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| Affiliation: | (1) Departments of Internal Medicine, University of Texas Health Science Center at Dallas, Southwestern Medical School, Harry Hines Boulevard, 5323 Dallas, Texas 75235, USA;(2) Microbiology, University of Texas Health Science Center at Dallas, Southwestern Medical School, 5323 Harry Hines Boulevard, 75235 Dallas, Texas, USA |
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| Abstract: | Previous studies have demonstrated restriction fragment length polymorphisms (RFLP) in the vicinity of the alpha and beta genes of the human T-cell receptor. In the course of experiments designed to discover additional polymorphic restriction sites, we found a new RFLP of the T-cell alpha gene recognized by the restriction enzyme Taq I. The site was localized to the interval between the most 3 joining (J) exon and the most 5 constant (C) region exon, about 7 kb distant from the previously described Bgl II polymorphic site which mapped to the vicinity of the 3 untranslated exon. With the use of these two polymorphic markers, four Ti-alpha alleles could be identified, allowing unambiguous assignment of all Ti-alpha genes in some families. These markers may be useful in identifying possible immune response genes or disease predisposition genes associated with the genes of the T-cell receptor for antigen.Abbreviations used in this paper RFLP restriction fragment length polymorphism - Ti-alpha alpha gene of the T-cell receptor for antigen |
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