A new linear DNA plasmid isolated from the yeast Saccharomyces kluyveri

Summary A new linear DNA plasmid, designated pSKL, was found in the yeast Saccharomyces kluyveri. Restriction maps of the 14.2 kb plasmid were constructed. By the use of CsCl-Hoechst 33258 centrifugation containing guanidine chloride, pSKL was isolated as a DNA-protein complex. The protein was associated with the terminal regions of pSKL. The two terminal EcoRI fragments of pSKL were cloned and their nucleotide sequences were determined. pSKL had inverted terminal repeats of 483 bp with a unique structure in which fairly homologous sequences of 30 bp were repeated eight times.