Purine nucleoside phosphorylase heterogeneity in the mouse as analyzed by isoelectric focusing and specific activity |
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| Authors: | E R Mably T Carter-Edwards F G Biddle F F Snyder |
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| Institution: | Department of Medical Biochemistry, University of Calgary, Alberta, Canada. |
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| Abstract: | 1. Isoelectric focusing in polyacrylamide gels identified three erythrocytic electrophoretic patterns of purine nucleoside phosphorylase in a survey of 16 mouse strains. 2. Three strain-specific electrophoretic types were also evident in liver, kidney and spleen leukocytes. 3. There are 3-fold differences in purine nucleoside phosphorylase activities between strains for several tissues; C57BL/6J and Mus spretus having the greatest and the least activity, respectively. 4. Within strains there were up to 8-fold tissue-specific differences in activity with the order from greatest to least being: liver, kidney, spleen leukocyte, erythrocyte, heart. |
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