The insensitivity of mushroom nuclear RNA polymerase activity to inhibiton by amatoxins |
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| Authors: | Paul A. Horgen Allan C. Vaisius Joseph F. Ammirati |
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| Affiliation: | (1) North American Poison Mushroom Research Centre, University of Toronto, L5L 1C6 Mississauga, Ontario, Canada;(2) Department of Botany, University of Toronto, L5L 1C6 Mississauga, Ontario, Canada |
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| Abstract: | Nuclear fractions isolated from Amanita phalloides, Amanita muscaria and Agaricus bisporus were subjected to in vitro RNA synthesis assays in the presence of various concentrations of amatoxins. The mushroom nuclei were highly insensitive to inhibition by amatoxin when compared to assays of nuclear fractions isolated from the Oömycete fungus, Achlya ambisexualis and from rabbit brain.Abbreviations DTT dithiothreitol - PMSF phenylmethyl sulfonyl fluoride - MES 2[N-morpholino] ethane sulfonic acidPaper no. 1-78 |
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