The efficacy of a commercial β-glucan preparation, EcoActiva™, on stimulating respiratory burst activity of head-kidney macrophages from pink snapper (Pagrus auratus), Sparidae |
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| Authors: | Pagrus auratus |
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| Institution: | a Department of Haematology, Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia, 5000;b Centre for Pharmaceutical Research, School of Pharmacy and Biomedical Sciences, University of South Australia, Frome Road, Adelaide, South Australia, 5000;c Aquaculture Program, Aquatic Sciences, South Australian Research and Development Institute, P.O. Box 120, Henley Beach, South Australia, 5022;d School of Aquaculture, University of Tasmania, Launceston, Tasmania, 7250 |
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| Abstract: | This study investigated the in vitro effects of a commercial β-glucan preparation, EcoActiva™, on the respiratory burst activity of head-kidney macrophages isolated from pink snapper (Pagrus auratus), a marine fish cultured in Australia. Macrophages incubated with EcoActiva™ displayed morphological characteristics of activation, and were stimulated to produce superoxide. Pre-incubation with low levels of EcoActiva™ significantly increased the response to phorbol myristate acetate (PMA) and lipopolysaccharide (LPS), indicating that EcoActiva™ could prime these macrophages. Co-culturing macrophages with both LPS and PMA, or EcoActiva™ and PMA, increased burst activity compared with the response to PMA alone, however, this increase was additive and not synergistic. These results suggest that EcoActiva™ is able to stimulate non-specific immunity in snapper through increased respiratory burst activity of macrophages, an important component of the host defence network. |
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| Keywords: | snapper Pagrus auratus respiratory burst PMA LPS β -glucan immunostimulation |
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