A method for protein extraction from different subcellular fractions of laticifer latex in <Emphasis Type="Italic">Hevea brasiliensis</Emphasis> compatible with 2-DE and MS期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2-DE and MS

Authors:	Xuchu Wang Minjing Shi Xiuli Lu Ruifeng Ma Chenggong Wu Anping Guo Ming Peng Weimin Tian

Affiliation:	(1) Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou Hainan, China;(2) Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou Hainan, China

Abstract:	Background Proteomic analysis of laticifer latex in Hevea brasiliensis has been received more significant attentions. However, the sticky and viscous characteristic of rubber latex as cytoplasm of laticifer cells and the complication of laticifer latex membrane systems has made it challenge to isolate high-quality proteins for 2-DE and MS.

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