| 蜻蜓凤梨花发育相关B类MADS-box基因克隆及表达分析 |
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| 引用本文： | 张俊芳,徐 立,李志英.蜻蜓凤梨花发育相关B类MADS-box基因克隆及表达分析[J].西北植物学报,2012,32(5):866-870. |
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| 作者姓名： | 张俊芳 徐 立 李志英 |
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| 作者单位： | 1. 海南大学农学院,海口570228;中国热带农业科学院热带作物品种资源研究所&农业部华南作物基因资源与种质创制重点实验室,海南儋州571737
2. 中国热带农业科学院热带作物品种资源研究所&农业部华南作物基因资源与种质创制重点实验室,海南儋州,571737 |
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| 基金项目： | 海南省自然科学基金（808191）；中国热带农业科学院博士启动基金（Pzsb0806,Rkyy0604）;中央级公益性科研院所基本科研业务费专项资金（PZS004） |
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| 摘 要： | 为探索MADS-box基因在凤梨花发育过程中的调控机制,通过设计简并引物,利用RACE技术,从蜻蜓凤梨花蕾中分离得到2个花发育相关B类MADS-box基因,分别命名为AfAP3和AfPI;AfAP3cDNA全长957bp,编码区编码226个氨基酸;AfPI cDNA全长808bp,编码区编码198个氨基酸,二者均具有典型的植物MADS-box蛋白结构.RT-PCR分析结果表明,AfAP3和AfPI基因主要在花器官中表达,在根系中也有微量表达;乙烯诱导后7d,AfPI基因在茎尖处开始有表达,表明此时蜻蜓凤梨花芽分化可能已经完成,AfAP3基因表达晚于AfPI.
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| 关 键 词： | 蜻蜓凤梨 MADS-box基因 RT-PCR |
Clonging and Expression Analysis of B Function MADS-box Genes Involved in Floral Development from Aechmea fasciata |
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| ZHANG Jun fang,XU Li,LI Zhi ying.Clonging and Expression Analysis of B Function MADS-box Genes Involved in Floral Development from Aechmea fasciata[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(5):866-870. |
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| Authors: | ZHANG Jun fang XU Li LI Zhi ying |
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| Institution: | 1 College of Agriculture,Hainan University,Haikou 570228,China;2 Institute of Tropical Crop Genetic Resources,Chinese Academy of Tropical Agricultural Sciences & Key Laboratory of South China Gene Resources and Germplasm Creating,Ministry of Agriculture,Danzhou,Hainan 571737,China) |
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| Abstract: | Two flowering-related MADS-box genes,named as AfAP3 and AfPI were cloned from flower bud of Aechmea fasciata through designing degenerate primer and RACE technology.The full cDNA fragment of AfAP3 was 957 bp and coded a polypeptide of 226 amino acids.The full cDNA fragment of AfPI was 808 bp and coded a polypeptide of 198 amino acids.Both genes had a typical MADS-box domain.RT-PCR analysis showed that these two genes were main expressed in floral organs;on the other hand,they were also expressed a little in root.After flower induction by exogenous ethylene,AfPI gene began to express in stem tip at 7 d,AfAP3 gene began to express later. |
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| Keywords: | Aechmea fasciata MADS-box gene RT-PCR |
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