Inhibition of DNA synthesis in permeabilized L cells by novobiocin |
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Authors: | A Nishio E M Uyeki |
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Affiliation: | Department of Pharmacology, The University of Kansas Medical Center, 39th St. and Rainbow Blvd. Kansas City, Kansas 66103, USA. |
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Abstract: | Novobiocin was equipotent in inhibiting DNA and RNA synthesis in cultured mouse L cells. It also suppressed in vitro DNA and RNA synthesis in permeabilized L cells and nuclei; 50 percent inhibition of DNA and RNA synthesis was obtained by 1 mM and 20 mM novobiocin, respectively. ATP antagonized the effect of novobiocin. Nalidixic acid had a weak inhibitory effect on in vitro DNA synthesis; 10 mM nalidixic acid showed 60 percent inhibition. ATP did not antagonize nalidixic acid. The inhibitory effect of novobiocin exceeded that of aphidicolin. These findings suggest a participation of a gyrase- and/or type II topoisomerase-like enzyme in the DNA replication machinery in L cells. |
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Keywords: | supported by NIEHS Grant No. ES-02046 and to whom all correspondence should be addressed. |
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