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Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland
Authors:Yeun-Jung Kim  Hyuk-Jae Kwon  Naoshi Shinozaki  Sadamitsu Hashimoto  Masaki Shimono  Sung-Won Cho  Han-Sung Jung
Institution:(1) Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, 134 Shinchon-Dong, Seodaemoon-Gu, Seoul, 120-752, Korea;(2) Cornea Center, Tokyo Dental College, Ichikawa, Japan;(3) Oral Health Science Center HRC7, Department of Pathology, Tokyo Dental College, Chiba, Japan
Abstract:The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. 5-Bromo-2′-deoxyuridine (BrdU) injection followed by a long chasing period permitted the identification of LRCs based on the slow-cycling characteristic. In order to confirm the accurate localization of LRCs, BrdU and SMG-specific markers, including aquaporin5, cytokeratin, and smooth muscle actin, were examined by double-immunofluoresence staining. We found that LRCs were distributed in the acinus, duct, myoepithelium, and connective tissue. Moreover, ABCG2 (a known stem cell marker) was used for the characterization of LRCs and the localization of cells as putative stem/progenitor cells. ABCG2-expressing cells were distributed in various regions of the SMG but did not co-localize with LRCs. We suggest that putative progenitor cells exist in various regions of the SMG and have diverse capacities to differentiate into specific cells. Yeun-Jung Kim and Hyuk-Jae Kwon contributed equally to this work. This work was supported by Korea Research Foundation Grant (KRF-2006–013-E00143).
Keywords:Label-retaining cells  BrdU  ABCG2  Progenitor cell  Submandibular gland  Mouse (ICR)
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