Correlation between HSP90 induction kinetics in murine leukemia cells and the amount of cisplatin over a wide range of cytostatic concentrations |
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Authors: | Detcheva Roumiana L Kenderov Andrey N Russinova Angelina I Spassovska Nadejda C Kolev Kolyo G Grancharov Konstantin C |
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Affiliation: | Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia. roumi@obzor.bio21.bas.bg |
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Abstract: | The induction of HSP90 in murine erythroleukemia cells, clone F4 N, by cisplatin (DDP) was examined using indirect immunofluorescence and avidin-biotin technique, and compared with cisplatin cytotoxicity. A reverse dependence of HSP90 induction time was found on a wide range of cisplatin concentrations (0.5-10 microM), which proved to be cytostatic up to 48 h of continuous treatment. Thus, the observed induction pattern of HSP90 in F4 N cells strictly correlated with their high tolerance toward DDP. This indicates that HSP90 might be responsible, at least in part, for cisplatin resistance of F4 N cells. |
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