L-type calcium current in right ventricular outflow tract myocytes of rabbit heart |
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Authors: | ShengHui Liang ChenHui Lin Yuan Li TaiFeng Liu Yan Wang |
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Institution: | 1. Fujian Medical University Graduate Student Education Institute, Fuzhou, 350004, China 2. Xiamen Heart Center, Xiamen Zhongshan Hospital of Xiamen University, Xiamen, 361004, China 3. Department of Physiology and Biophysics, College of Life Sciences, Peking University, Beijing, 100871, China
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Abstract: | The mechanism of idiopathic ventricular tachycardia originating from the right ventricular outflow tract (RVOT) is not clear.
Many clinical reports have suggested a mechanism of triggered activity. However, there are few studies investigating this
because of the technical difficulties associated with examining this theory. The L-type calcium current (I
Ca-L), an important inward current of the action potential (AP), plays an important role in arrhythmogenesis. The aim of this
study was to explore differences in the APs of right ventricular (RV) and RVOT cardiomyocytes, and differences in electrophysiological
characteristics of the ICa-L in these myocytes. Rabbit RVOT and RV myocytes were isolated and their AP and I
Ca-L were investigated using the patch-clamp technique. RVOT cardiomyocytes had a wider range of AP duration (APD) than RV cardiomyocytes,
with some markedly prolonged APDs and markedly shortened APDs. The markedly shortened APDs in RVOT myocytes were abolished
by treatment with 4-AP, an inhibitor of the transient outward potassium current, but the markedly prolonged APDs remained,
with some myocytes with a long AP plateau not repolarizing to resting potential. In addition, early afterdepolarization (EAD)
and second plateau responses were seen in RVOT myocytes but not in RV myocytes. RVOT myocytes had a higher current density
for I
Ca-L than RV myocytes (RVOT (13.16±0.87) pA pF−1, RV (8.59±1.97) pA pF−1; P<0.05). The I
Ca-L and the prolonged APD were reduced, and the EAD and second plateau response disappeared, after treatment with nifedipine
(10 μmol L−1), which blocks the I
Ca-L. In conclusion, there was a wider range of APDs in RVOT myocytes than in RV myocytes, which is one of the basic factors involved
in arrhythmogenesis. The higher current density for I
Ca-L is one of the factors causing prolongation of the APD in RVOT myocytes. The combination of EAD with prolonged APD may be
one of the mechanisms of RVOT-VT generation. |
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